Chlamydiaceae

Chlamydiaceae | Microbix Biosystems

Product Features

Name & Catalogue No. Description Applications
Chlamydia trachomatis EL-12-02-001 (1 mL) EL-12-02-010 (10 mL)
  • Chlamydia trachomatis LGV type II strain 434 is cultured in murine L cells; cellular debris is removed via centrifugation and the resulting preparation resuspended in PBS
  • Positive control in Chlamydia antigen detection assays
  • ELISA for antibody detection
Chlamydophila pneumoniae EL-46-02-001 (1 mL) EL-46-02-010 (10 mL)
  • Strain CWL-029 is cultured in HL cells
  • Microbix has a proprietary production process allowing for optimal yield of C. pneumoniae elementary bodies.
  • The final preparation is resuspended in SPG buffer and gamma irradiated
  • ELISA
  • Western Blot
 
Chlamydophila pneumoniae, still commonly referred to as Chlamydia pneumoniae, was sequenced in 1999. Further sequencing of related species lead to the proposal of a new taxonomy for the Chlamydiaceae family. The result was the creation of the genus Chlamydophila alongside the genus Chlamydia. However, there remains some debate between chlamydiologists about the reclassification including a proposal to reunite the organisms under a single genus.

Other Information

Quality Assurance
Each antigen lot is tested for potency using ELISA, relative to a reference antigen. This ensures the amount of antigen in each batch is consistent. The total protein concentration reported includes both chlamydia and non-chlamydia proteins, with the ratio dependent on the purity of the antigen.

Please click here for Microbix’ Quality Policy and ISO Certification.

Product Images

Chlamydia trachomatis infection in L-Cells

A monolayer of McCoy cells infected with C. trachomatis Serovar D. Inclusion forming units (IFU) identified by use of a chemiluminescence immunoassay, which stains internal elementary bodies (EB’s) using diaminobenzidine (DAB) and identifies the IFU with a brown stain.

Inactivation and Safety
Each lot of Chlamydiaceae antigen is inactivated using gamma radiation and this inactivation verified using a sensitive tissue culture based infectivity assay.
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Product Information Sheet
Frequently Asked Questions

1. Does Microbix offer other types of Chlamydia?

Yes, we culture other species and serovars of Chlamydia on a custom basis. Please contact customer service if you have specific requirements.

2. Will EL-12-02 cross react with other Chlamydia species?

Yes. The genus Chlamydia comprises three species,  C. trachomatis,  C. muridarum,  and C. suis. All share a common genus-specific lipopolysaccharide (LPS) antigen that is further shared by members of the genus Chlamydophila, in addition to  crossreactive outer membrane protein antigens. Due to the presence of such common antigenic determinants, EL-12-02 will cross-react with other Chlamydiaceae species.

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Ordering Information
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