Rubella

Rubella | Microbix Biosystems

Product Features

Name & Catalogue No. Description Applications  
Rubella KOS Concentrate EL-05-13-001 (1 mL) EL-05-13-010 (10 mL)
  • Concentrated viral lysate
  • IgM capture formats
  • Some solid phase IgG tests
 
Rubella K1S EL-05-10-001 (1 mL) EL-05-10-010 (10 mL)
  • Concentrated viral lysate is purified via sucrose density gradient centrifugation, providing a semi-purified antigen of >60% viral protein
  • Many types of IgG and IgM immunoassays
 
Rubella K2S EL-05-11-001 (1mg) EL-05-11-005 (5mg)
  • Our top selling product, high purity rubella is the industry gold standard
  • Concentrated viral lysate is purified via 2 rounds of sucrose density gradient centrifugation, providing a highly purified antigen of >90% viral protein
  • Suitable for all IgG and IgM immunoassays
  • Direct coating or conjugation to latex, polystyrene, or gold particles
 
Every rubella product from Microbix contains all major immunogens of the rubella virus (E1, E2, and capsid proteins). We culture the virus under serum-free conditions which facilitate production of highly purified viral antigen.

Additional Information

Quality Assurance
Each antigen lot is tested for potency using ELISA, and compared to a reference antigen. This ensures the amount of viral protein in each batch is consistent. The total protein concentration reported includes both viral and non-viral proteins, with the ratio dependent on the purity of the antigen.

Please click here for Microbix’ Quality Policy and ISO Certification.

Product Images

Rubella K2S Western Blot

Western Blot analysis of Microbix’ K2S antigen from 5 consecutive manufacturing campaigns. Western Blot was developed using rubella- positive human patient serum. Banding pattern is consistent with the presence of viral proteins only; no extraneous protein is detectable.

Inactivation and Safety
Each lot of Rubella antigen is inactivated using  ultraviolet radtiation and this inactivation verified using a sensitive tissue culture based infectivity assay.

Microbix’ proprietary inactivation strategy is the best in the industry, and provides the highest level of safety you will find.

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Product Information Sheet
Frequently Asked Questions

How do I choose between the 3 grades of rubella?

All three rubella antigens are suitable for commonly used immunoassays. Typically selection of the antigen will depend on the application and the specific assay set up.

Rubella K0S antigen is suitable for assays that can tolerate a large amount of the antigen in the assay. It is a cost-effective antigen that could be suitable for initial and investigative stages of the assay development. This product contains extraneous protein that could compete with rubella antigen for binding to analytes or solid support. It is not recommended for assays or applications which require direct covalent coupling of the antigen to another entity.

Rubella K1S antigen is purified by sucrose gradient centrifugation and ratio of rubella antigen to extraneous protein is significantly higher than for Rubella K0S antigen. As such it can be used in any assay set up including assays that require direct covalent conjugation of the antigen, with appreciable conjugation efficiency.

Rubella K2S as highly purified product contains rubella virus only and no extraneous protein. As such this antigen is ideal for assays that require highest level of sensitivity and specificity. Due to the high purity level of Rubella K2S antigen, the antigen can be used for conjugation to other entities such as other proteins (e.g. antibodies, enzymes) or to solid support and particles (latex, iron, nanogold) with maximum conjugation efficiency. Generally, Rubella K2S antigen is used at a high dilution due to the high purity of the antigen.

For additional FAQs, click through to our FAQ Page.

Ordering Information
To find out more about how to order this product, proceed to our order section.

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