How pure are your antigens, and how are they purified?
Microbix offers a variety of antigen preparations with different characteristics. These range from clarified lysates to partially purified antigens to highly purified viruses or single proteins. Contact us with your assay specifications so that our application scientists can recommend the purity that meets your needs.
Can you explain the difference between Grade 1, Grade 2, etc.?
In many cases, Microbix manufactures antigen preparations with different characteristics suitable for specific purposes. These are often differentiated as “Grades” of antigen. The culture, purification, and inactivation methods and resulting product differ in case-specific ways. A rule of thumb is purity increases as the Grade Number increases. For example, Rotavirus Grade 3 has a higher purity than Rotavirus Grade 2. We recommend you speak with your Customer Service representative for details on each product and advice on which is right for your application.
Are the antigens inactivated, and if so how do you confirm this?
Microbix’ antigens are inactivated by physical or chemical treatment. Confirmation of this is found on the Certificate of Analysis accompanying each shipment. Microbix employs a range of inactivation techniques including gamma and ultraviolet radiation, beta-propiolactone, and formaldehyde. After the inactivation treatment, a sensitive inactivation assay is performed on each lot. These assays are carefully designed to amplify any residual infectious particles; if no growth is detected on susceptible cells it proves the lot is certified inactivated and released.
Can Microbix produce virus and cells on a custom basis?
Microbix has expertise in producing a large variety of cells and viruses. Please contact our customer service department with your requirements.
How are antigens shipped? Do I need any additional paperwork to import your antigens from Canada?
Antigens are shipped in dry ice and are packaged in accordance with all international shipping and labeling regulations.
The antigens are non-hazardous, and as such do not require specific importation documentation to enter the USA and most other countries. To make things easier for our US clients, Microbix ships our antigens from Buffalo, New York Monday through Wednesday, ensuring overnight service and no customs delays.
How should the antigens be stored?
Microbix recommends that most of our antigens be stored frozen at -70°C. It is best to avoid thawing and refreezing of material. Final dilutions should be made just prior to use.
A few products have alternative storage recommendations which will be discussed with anyone ordering these items.
Will the antigens work in ELISA?
All Microbix antigens are designed specifically for ELISA (EIA) systems. We recommend that titrations be performed to determine the optimum dilutions in your system.
Will the antigens work in Molecular Assays?
Most of our antigens have been used successfully in molecular tests.
Will the antigens work in Western blot?
Most Microbix antigens are suitable for Western Blot.
Will the antigens work in cellular immunity assays?
Many of our antigens are currently used successfully in a range of lymphoproliferation assays (ELISPOT assays, for intracytoplasmic cytokine production, for the generation of established CD4 T cell clones, etc).
Will the antigens work in IFA?
Generally, purified antigens are not suitable for establishing IFA slides.
How is the protein concentration determined?
Protein is determined using the Bio-Rad dye binding assay in the microassay format. The standard curve is generated with a known concentration of IgG.