LumiSort™ is Microbix’ next generation semen sexing technology for the livestock industries. Quality sexed semen is critical to the efficiency of herd management and genetic improvement, and will be an important contributor to productivity and global food security in the coming years. LumiSort will be a key contributor to global efforts for efficiency in this vital sector. The technology will enable the widespread use of semen sexing by providing significant improvements to the yield and quality of sexed semen, while greatly increasing the speed of sperm cell sorting.
LumiSort technology incorporates a novel and innovative instrument design to address fundamental problems with the existing commercial sex selection technology. LumiSort’s innovations provide Microbix with a strong and well-differentiated intellectual property advantage in the industry.
The LumiSort sperm sexing method couples a pioneering optical system for the detection of sperm cell sex, with a fast and effective laser-based killing method that eliminates the sperm cells that are not of the desired sex. In this way, livestock reproductive specialists can select sperm cells that will give rise to offspring of the desired sex, which permits reproduction for both herd replacement and for genetic selection to proceed with maximum efficiency. Click on the video below to see a conception of the LumiSort cytometer (sperm cell sorter) in operation.
The LumiSort instrument will achieve sperm cell sexing with unparalleled speed and efficiency by performing four basic operations. First, cells are positioned accurately and single file in a rapidly flowing fluid. The cells then pass into the analysis region of the instrument where they are illuminated with a light that causes them to glow brightly, taking advantage of a special fluorescent dye that permits the discrimination of female-determining sperm cells (slightly brighter) from male-determining cells (slightly dimmer). Next, the emitted light is collected by the LumiSort instrument’s sensitive light collection optics, taking advantage of 360-degree light collection to overcome a problem caused by the cells’ uneven light emission in different directions. With the sex determined, the fast electronics in the LumiSort instrument will control the delivery of lethal laser irradiation to a spot downstream of the analysis region, timed to coincide with the passage of sperm cells of the undesired sex. The cells of the desired sex pass through the instrument unharmed and are collected for processing, packaging, transportation and use in artificial insemination.