Semi-purified viral lysate containing a high titre of HSV-2 viral particles. Can be used for IgG and IgM ELISA, Western Blot, and Lymphocyte proliferation assays.
Herpes simplex virus (HSV) is a double stranded DNA, enveloped virus, 110 nm to 120 nm in diameter. The icosahedral viral capsid is assembled in the host cell nucleus followed by the virus acquiring its envelope by budding through the nuclear membrane at sites modified by the addition of viral protein. The HSV genome encodes over 50 polypeptides, including 9 glycoproteins (gB to gJ), at least 6 capsid proteins, viral protein kinase and DNA polymerase. There are two serotypes, HSV type 1 and HSV type 2 that can be differentiated on the basis of antigenicity of glycoprotein gG. The serotypes have approximately 40% homology leading to antigenic cross reactivity.
Inactivation & Safety — Each lot of HSV antigen is inactivated using gamma radiation and this inactivation verified using a sensitive tissue culture based infectivity assay.
P3H3 Cell extract and EBV-VCA gp125 are prepared using detergents that are documented to inactivate any residual viral activity.
Applications — IgG and IgM ELISA, Western Blot, Lymphocyte proliferation assays