|Name & Catalogue No.||Description||Applications|
|Dengue 2 Antigen EL-22-02-001 (1 mL) EL-22-02-010 (10 mL)||
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Inactivation and Safety
Product Information Sheet (sample)
Frequently Asked Questions
Will Dengue 2 antigen react with serum antibodies from Dengue 1, 3, and 4?
Yes, Dengue 2 antigen reacts with serum antibodies from Dengue 1, 3, and 4 due to natural cross-
reactivity. For example, the envelope protein E is major immunogen and main target of the immune
response. Common genetic determinants of the envelope protein E give rise to marked antibody cross-reactivity between all Dengue serotypes.
What is medium 199?
Medium 199 is a medium that is used in cell biology for culturing cells. This is the storage buffer used for EL-22-02.
Do I need to dialyze the antigen prior to conjugation with gold?
Medium 199 contains amino acids and other additives. Thiol containing compounds such as amino acid cysteine can interfere with conjugation by binding to nanogold thereby inhibiting conjugation of virus to nanogold. Removing cysteine form conjugation reaction mixture (e.g. by dialysis) could improve conjugation efficiency.
Is your Dengue 2 antigen prepared as a lysate or as a whole virus?
Dengue 2 antigen is prepared by sucrose gradient centrifugation. As such it contains whole virus, although it is possible that there is small amount of free protein present in a sample. If the goal of conjugation is conjugation of whole virus to nanogold particle, no lysis is necessary. If the goal of conjugation is conjugation of lysates, then Dengue 2 antigen can be lysed with detergents.
I have lysed Dengue with detergent. Do I need to remove the detergent prior to conjugation?
Yes, generally you will need to remove detergent by dialysis or by some other method. A small amount of some detergents can actually improve conjugation efficiency.