Dengue

Dengue Mosquito | Microbix Biosystems

Product Features

Name & Catalogue No. Description Applications
Dengue 2 Antigen EL-22-02-001 (1 mL) EL-22-02-010 (10 mL)
  • Concentrated viral lysates purified through sucrose gradient
  • Final antigen comprised of intact virions plus free viral proteins and nonstructural proteins
  • NS1 and E proteins present in good quantity
  • IgG and IgM detection assays including ELISA with polystyrene and latex solid phases
  • Western Blot
  • Lateral flow assays
  • The antigen is suitable for conjugation to gold or latex particles
Microbix is currently developing purified native Dengue 1, 3 and 4 antigens, as well as specific membrane and nonstructural proteins. Check back for updates on our progress on this major project. 

Other Information

Quality Assurance
Each antigen lot is tested for potency using ELISA, and compared to a reference antigen. This ensures the amount of viral protein in each batch is consistent. The total protein concentration reported includes both viral and non-viral proteins, with the ratio dependent on the purity of the antigen.

For more information, please visit our Quality Assurance page.

Product Images

Dengue Western

Dengue Gel

Inactivation and Safety
Each lot of dengue antigen is inactivated chemically and this inactivation verified using a sensitive tissue culture based infectivity assay. This product contains no detectable infectious material
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Product Information Sheet (sample)
Frequently Asked Questions
      1. Will Dengue 2 antigen react with serum antibodies from Dengue 1, 3, and 4?

        Yes, Dengue 2 antigen reacts with serum antibodies from Dengue 1, 3, and 4 due to natural cross-
        reactivity. For example, the envelope protein E is major immunogen and main target of the immune
        response. Common genetic determinants of the envelope protein E give rise to marked antibody cross-reactivity between all Dengue serotypes.

      2. What is medium 199?

        Medium 199 is a medium that is used in cell biology for culturing cells. This is the storage buffer used for EL-22-02.

      3. Do I need to dialyze the antigen prior to conjugation with gold?

        Medium 199 contains amino acids and other additives. Thiol containing compounds such as amino acid cysteine can interfere with conjugation by binding to nanogold thereby inhibiting conjugation of virus to nanogold. Removing cysteine form conjugation reaction mixture (e.g. by dialysis) could improve conjugation efficiency.

      4. Is your Dengue 2 antigen prepared as a lysate or as a whole virus?

        Dengue 2 antigen is prepared by sucrose gradient centrifugation. As such it contains whole virus, although it is possible that there is small amount of free protein present in a sample. If the goal of conjugation is conjugation of whole virus to nanogold particle, no lysis is necessary. If the goal of conjugation is conjugation of lysates, then Dengue 2 antigen can be lysed with detergents.

      5. I have lysed Dengue with detergent. Do I need to remove the detergent prior to conjugation?

        Yes, generally you will need to remove detergent by dialysis or by some other method. A small amount of some detergents can actually improve conjugation efficiency.

For additional FAQs, click through to our FAQ Page.

Ordering Information
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