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Semipurified rotavirus inactivated by gamma irradiation.

Taxonomy: Rheoviridae/Rotavirus/Rotavirus A/Simian rotavirus SA11. Virions consist of a capsid, a core, and a nucleoprotein complex. Virus capsid is not enveloped. Capsid/nucleocapsid is isometric with icosahedral symmetry and has a diameter of 80 nm. The genome is segmented and consists of eleven segments of linear double-stranded RNA. Rotavirus is the most common cause of severe diarrhea among children. The incubation period for rotavirus disease is approximately 2 days. The disease is characterized by vomiting and watery diarrhea for 3 – 8 days, and fever and abdominal pain occur frequently.

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Inactivation & Safety — Each lot of rotavirus antigen is inactivated using gamma or ultraviolet radiation and this inactivation verified using a sensitive tissue culture based infectivity assay.

Applications — Positive control in lateral flow (rapid) assays. IgG and IgM detection in immunoassays including EIA with polystyrene, latex, nanogold and/or iron solid phases using different detection systems (colorimetric, chemiluminescent.


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