Chlamydia trachomatis LGV type II strain 434 is cultured in murine L cells; cellular debris is removed via centrifugation and the resulting preparation resuspended in PBS.
Chlamydia trachomatis is a non-motile gram negative intracellular bacterium, which has two forms during replication. The extracellular form, used in antigen preparations, is the elementary body. It has a diameter of 0.25 – 0.35 µm. The intracellular form is the reticulate or initial body which is 0.25 – 1 µm in diameter. Although Chlamydia trachomatis is antigenically complex only a few antigens are important in diagnostic testing. Sixty percent of the dry weight of the outer membrane of the elementary body is composed of major outer membrane protein (MOMP). MOMP is a transmembrane protein with type, species and genus reactive epitopes. It possesses an epitope used to characterize C. trachomatis into 15 serotypes or serovars. The outer membrane of C. trachomatis has a heat stable lipopolysaccharide antigen (LPS) with 2-keto-3-deoxyoctonic acid as an immunodominant component. This antigen contains the reactive epitopes employed in genus specific serological tests. There is structural similarity and antigenic cross reactivity between chlamydial and other gram negative LPS moieties.
Inactivation & Safety — Each lot of Chlamydiae antigen inactivated using gamma radiation and this inactivation verified using a sensitive tissue culture based infectivity assay.
Applications — Positive control in Chlamydia antigen detection assays. ELISA for antibody detection.